Blood Parasites

Welcome to Blood Parasites

Blood Parasites Resources

Home > Blood Parasites > The human ikkbeta subunit kinase domain displays ck2-like phosphorylation specificity.

The human ikkbeta subunit kinase domain displays ck2-like phosphorylation specificity.

The human IKKbeta subunit kinase domain displays CK2-like phosphorylation specificity.

Biochem Biophys Res Commun. 2008 Sep 26;374(3):592-7

Authors: Shaul JD, Farina A, Huxford T

NF-kappaB activation in response to pro-inflammatory stimuli relies upon phosphorylation of IkappaB alpha at serines 32 and 36 by the beta subunit of the IkappaB kinase complex (IKK). In this study, we build upon the observation that highly purified human IKKbeta subunit preparations retain this specificity in vitro. We show that IKKbeta constructs that lack their carboxy-terminus beginning at the leucine zipper motif fail to phosphorylate IkappaB alpha at Ser-32 and Ser-36. Rather, these constructs, which contain the entire IKKbeta subunit kinase domain, phosphorylate serine and threonine residues contained within the IkappaB alpha carboxy-terminal PEST region. Furthermore, removal of the leucine zipper and helix-loop-helix regions converts IKKbeta to monomer. We propose that the helix-loop-helix of the human IKKbeta subunit is necessary for restricting substrate specificity toward Ser-32 and Ser-36 in IkappaB alpha and that in the absence of its carboxy-terminal protein structural motifs the human IKKbeta subunit kinase domain exhibits a CK2-like phosphorylation specificity.

PMID: 18657515 [PubMed - indexed for MEDLINE]

(Source: Biochemical and Biophysical Research communications)

Click Here to read the entire article...

Other Articles:

•	Ben-Gurion University of the Negev initiates project to eliminate intestinal worms in Ethiopia
	A professor at The Faculty of Health Sciences at Ben-Gurion University of the Negev (BGU) is beginning an intensive program in Ethiopia this August to eradicate intestinal worms which affect as much as 50 percent of the population in Africa. (2008-08-06)
	Click Here to read the entire article...

•	Human stem cells help brain-impaired mice
	Injecting human stem cells into the brains of mice helped them recover almost fully from a neurological condition similar to a group of childhood diseases in people, researchers said.
	Click Here to read the entire article...

•	Mustard - hot stuff for natural pest control
	Researchers, growers and Industry specialists from 22 countries will share the latest research into the use of Brassica species, such as mustard, radish, or rapeseed, to manage soil-borne pests and weeds - a technique known as biofumigation. (2008-07-28)
	Click Here to read the entire article...

•	How Does Bluetongue Virus Survive Through The Winter?
	In 2006, Bluetongue virus -- which infects livestock -- reached Northern Europe for the first time. Some people thought that the outbreak would be limited to that particular year, as winter was expected to kill off the midges that host and spread the disease, bringing the threat of infection to an end. In actuality, the disease escalated in the following year, spreading to the UK. So, how did the virus survive the winter?
	Click Here to read the entire article...

•	Create Big Profits Online
	35000+ Recruiters, 200000+ Jobs Apply Free Fast! http://always-young.synthasite.com/
	Click Here to read the entire article...

•	Birth characteristics and the risk of childhood leukaemias and lymphomas in new zealand: a case-control study
	Background: Some studies have found that lower parity and higher or lower social class (depending on the study) are associated with increased risks of childhood acute lymphoblastic leukaemia (ALL). Such findings have led to suggestions that infection could play a role in the causation of this disease. An earlier New Zealand study found a protective effect of parental marriage on the risk of childhood ALL, and studies elsewhere have reported increased risks in relation to older parental ages. This study aimed to assess whether lower parity, lower social class, unmarried status and older parental ages increase the risk of childhood ALL (primarily). These variables were also assessed in relation to the risks of childhood acute non-lymphoblastic leukaemia, non-Hodgkins lymphomas and Hodgkins disease. Methods: A case control study was conducted. The cases were 585 children diagnosed with leukaemias or lymphomas throughout New Zealand over a 12 year period. The 585 age and sex matched controls were selected at random from birth records. Birth records from cases (via cancer registration record linkage) and from controls provided accurate data on maternal parity, social class derived from paternal occupation, maternal marital status, ages of both parents, and urban status based on the address on the birth certificate. Analysis was by conditional logistic regression. Results: There were no statistically significant associations overall between childhood ALL and parity of the mother, social class, unmarried maternal status, increasing parental ages (continuous analysis), or urban status. We also found no statistically significant associations between the risks of childhood acute non-lymphoblastic leukaemia, non-Hodgkin lymphomas, or Hodgkins disease and the variables studied. Conclusions: This study showed no positive results though of reasonable size, and its record linkage design minimised bias. Descriptive studies (eg of time trends of ALL) show that environmental factors must be important for some diagnoses. Work has been done on the risk of ALL in relation to chemicals (eg pesticides) and drugs, dietary factors (eg vitamins), electromagnetic fields and infectious hypotheses (to name some); but whether these or other unknown factors are truly important remains to be seen. (Source: BMC Blood Disorders)
	Click Here to read the entire article...